Table 3 Primers for real-time PCR amplification.
Gene | GenBank No. | Primer | Primer sequence (5′~3′) | Amplicon size (bp) |
|---|---|---|---|---|
StAR | AY877430 | Sense | CGAGAGTGCCAATGGTGATAAGGTG | 93 |
Anti-sense | TCTGTCTTCTGTTCCAGCATCACTTCC | |||
P450c17 | FJ809936 | Sense | CCGACCTGTCGCCCCACT | 193 |
Anti-sense | GCAGCACAAACCATCACCCTC | |||
P450arom A | AB009336 | Sense | TGCTGGGTCTGGGTCCTCTT | 102 |
Anti-sense | CCCGCACAATGTCTCCGTAT | |||
P450scc | JQ340311 | Sense | GAGGCTTGGGACGGCATCTTTA | 107 |
Anti-sense | GCCAGCACACCAGGGTACTTCT | |||
3-beta-HSD | JQ867353 | Sense | TGCGAAGAGCGTGTAAAGGAGC | 193 |
Anti-sense | GGATTGGGACCAGCCACCTC | |||
17-beta-HSD1 | JX036034 | Sense | TGCTGTCCATCTCGCATCAAAT | 163 |
Anti-sense | GTCAAGTATAGACTGCTGGTCGGTC | |||
20-beta-HSD | KC193327 | Sense | TGCGGTTGCTGGACTGAAATCTG | 173 |
Anti-sense | GCTCTGTTGCTGAATGTTTGAAGGC | |||
P45011beta | KC193328 | Sense | ATCCTGGGTGTGCTGGGTCAG | 145 |
Anti-sense | GGTTCCTCGCTAACTCGAAGAGAG | |||
11-beta-HSD2 | KC193326 | Sense | AGGTAGACATCACCCAGCCTCAGC | 114 |
Anti-sense | TGTTCACACACCATCCAGCGTTATT | |||
beta-actin | AB039726 | Sense | GAAACTGGAAAGGGAGGTAGC | 115 |
Anti-sense | CTGTGAGGGCAGAGTGGTAGA | |||
18S rRNA | FJ710820 | Sense | CGAGACGAGCCACCACCTATC | 148 |
Anti-sense | CGGTATTCAGCGGCGACAG |
