Figure 4 | Scientific Reports

Figure 4

From: Inhibition of Jak/STAT signaling reduces the activation of pancreatic stellate cells in vitro and limits caerulein-induced chronic pancreatitis in vivo

Figure 4

Jak/STAT inhibition reduces PSC activation in vitro. (a) Lysates of PaSC were taken after 72 hours of treatment with either ruxolitinib or MEK162. α-SMA expression was assessed by immunoblot and quantified by densitometric analysis. Graphs display mean ± SD of 3 biological replicates (* indicates p < 0.05). (b,c) PaSC were stained with Oil-Red O to examine quiescence or pseudoquiescence following incubation with vehicle, 10 μM ATRA (positive control), 10 μM ruxolitinib, or 10 μM MEK162 for 48 hours. (b) Light microscopy images were taken at 20X magnification. (c) Increased magnification of 20X images are shown below at 40X.

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