Figure 4
From: Structural studies of the periplasmic portion of the diguanylate cyclase CdgH from Vibrio cholerae
Structural comparison between the PBPb-I and PBPb-II domains. (a) Structural superposition of the PBPb-I and PBPb-II domains. The regions affecting the ligand-binding pocket of the PBPb-II domain are shown in the red rectangle. The opening angles for the PBPbs are indicated. Lobe-I and lobe-II of the PBPb-I domain are shown in green and yellow, respectively, while lobe-I′ and lobe-II′ of the PBPb-II domain are shown in cyan and orange, respectively. The L-arginine bound in the ligand-binding pocket is shown in magenta. (b) Ribbon representation of the closed PBPb-I and open PBPb-II domains. The cavities are shown in light magenta. (c) Superposition of the key ligand-binding residues of the PBPb-II domain (cyan) and PBPb-I domains (green). (d) Data from ITC experiments involving PBPb-IE56A/R109A/E122A/PBPb-II with L-glutamine and L-histidine.
