Figure 5

The dimerization of the periplasmic portion of CdgH. (a) The SV-AUC results of the wild-type CdgH (46–491), refolded CdgH (46–491), refolded CdgH (46–491) with L-arginine, and the wild-type PBPb-I domain. The calculated molecular mass of the samples are indicated beside the peaks. (b) Top view and side view of the type-I dimeric assembly of CdgH (46–491). (c) The overall structure of the type-II dimeric assembly of CdgH (46–491). The PBPb-I and -II domains of molecule A are shown in green and light green, respectively, and those of molecule B are shown in blue and light blue, respectively. (d) Comparison of the dimeric assemblies of CdgH and BvgS. The cartoon model of the GGDEF domain is generated from the structure of a zinc-sensory diguanylate cyclase from E. coli (PDB ID: 4H54). Substrate analog GTPαS molecules are shown as orange sticks. The cartoon model of the tandem periplasmic domains of BvgS is generated from the structure of BvgS (PDB ID: 4Q0C). The cartoon model of PAS (Per-Arnt-Sim) and HK (Histidine Kinase) domains of BvgS is generated from the structure of VicK from Streptococcus mutans (PDB ID: 4I5S). The transmembrane regions of both models are indicated by dash lines.