Figure 1 | Scientific Reports

Figure 1

From: Three-dimensional scoring of zebrafish behavior unveils biological phenomena hidden by two-dimensional analyses

Figure 1

Schematics of the setup to acquire 2D and 3D trajectories in response to the experimental stimuli. (a) Representative image of the 3D reconstructed trajectory obtained through the synchronization of 2D data from top (left) and front (right) views. (b) Images of the stimuli utilized in the six experimental conditions comparing individual preference for an empty compartment with preference for a compartment containing one of the following stimuli: live conspecific (LC); live predator (LP), red tiger oscar fish; predator model (PM), 3D-printed model of red tiger oscar fish anchored in the stimulus compartment and beating its tail through an external motor; computer-animated predator (CAP), animated image of red tiger oscar fish projected on a computer screen; conspecific model (CM), 3D-printed model of zebrafish actuated by a robotic platform to move along realistic 3D trajectories and oscillate its body; empty compartment, control (CTRL).

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