Figure 2 | Scientific Reports

Figure 2

From: Arginine-rich cell-penetrating peptide-modified extracellular vesicles for active macropinocytosis induction and efficient intracellular delivery

Figure 2

Enhanced cellular uptake efficiency of EVs upon modification with oligoarginine peptides. (a) Relative cellular uptake of CD63-GFP EVs (20 μg/ml) modified with Rn-EMCS (n = 4, 8, 12: 20 μM, n = 16: 10 μM) in CHO-K1 cells for 24 h at 37 °C according to a flow cytometry analysis. The data are expressed as the average (±SD) of three experiments. **p < 0.01, ***p < 0.001. (b) Confocal microscopy observations of CHO-K1 cells treated with FITC-dextran-encapsulated EVs (20 μg/ml) modified with Rn-EMCS (n = 8: 20 μM, n = 16: 10 μM) under the same experimental conditions as (a) (green: FITC-dextran-encapsulated EVs, blue: Hoechst 33342). Scale bar: 20 μm.

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