Table 2 Permutational multivariate analysis of variance (PERMANOVA) results for comparisons of FA concentration by population (PO), Species (SP), Age (AG), and their interactions.

From: Ecological relevance of skeletal fatty acid concentration and composition in Mediterranean scleractinian corals

Source

df

SS

MS

Pseudo-F

P(perm)

P(MC)

First model

 SP

3

8.10 × 10−02

2.70 × 10−02

17.968

0.001*

0.001*

 PO

1

1.14 × 10−03

1.14 × 10−03

0.76166

0.401

0.402

 AG

1

7.43 × 10−02

7.43 × 10−02

49.466

0.001*

0.001*

 SP × PO

3

2.14 × 10−02

7.14 × 10−03

4.7526

0.007*

0.007*

 SP × AG

3

4.23 × 10−02

1.41 × 10−02

9.3738

0.001*

0.002*

 PO × AG

1

7.14 × 10−04

7.14 × 10−04

0.47525

0.512

0.498

 SP × PO × AG

3

9.36 × 10−03

3.12 × 10−03

2.0759

0.114

0.107

 Res

31

4.66 × 10−02

1.50 × 10−03

   

 Total

46

0.28122

    

Second model

 SP

2

7.29 × 10−02

3.64 × 10−02

26.754

0.001*

0.001*

 PO

2

1.03 × 10−02

5.17 × 10−03

3.7924

0.041*

0.027*

 AG

1

6.24 × 10−02

6.24 × 10−02

45.819

0.001*

0.001*

 SP × PO

4

2.62 × 10−02

6.54 × 10−03

4.8022

0.007*

0.003*

 SP × AG

2

6.63 × 10−02

3.31 × 10−02

24.331

0.001*

0.001*

 PO × AG

2

2.53 × 10−03

1.27 × 10−03

0.93014

0.413

0.395

 SP × PO × AG

4

3.53 × 10−03

8.82 × 10−04

0.64777

0.621

0.636

 Res

34

4.63 × 10−02

1.36 × 10−03

   

 Total

51

0.29474

    
  1. Tests were run using euclidean distances among samples and 999 permutations in the software Primer®, including the Montecarlo correction for small sample size. The first Permanova model considered only the populations where all species were found and included the effects of the factors AG (two levels: young, old), PO (two levels: Palinuro and Pantelleria), SP (four levels: C. caespitosa, B. europaea, A. calycularis, L. pruvoti), and their interactions. The second Permanova model considered only the species that were found in all populations and included the effects of the factors AG (two levels: young, old), PO (three levels: Calafuria, Palinuro, Pantelleria), SP (three levels: C. caespitosa, B. europaea, L. pruvoti), and their interactions. df degrees of freedom, SS sum of squares, MS mean squares, P(perm) significance, P(MC) significance after Montecarlo correction. Significant effects (p < 0.05) are indicated with an asterisk (*).