Figure 3
From: Structure and function of a broad-specificity chitin deacetylase from Aspergillus nidulans FGSC A4
Effect of CBP21 on deacetylation of β-chitin by AnCDA. 1 mg/mL β-chitin was incubated with 1 μM AnCDA (diamonds) or with 1 μM AnCDA and 1 μM CBP21 (triangles) in 50 mM Tris-HCl, pH 8.0 containing 250 µM CoCl2 and 1 mM ascorbic acid at 37 °C. In a control reaction with only 1 μM CBP21 no acetate release was observed. Note that acetate release after 24 hours in the absence of CBP21 corresponds to approximately 0.5% deacetylation.
