Figure 6
Effects of four hybrid peptides on the inflammatory factors: (A) iNOS & nitrite; (B) TNF-α; (C) IL-1β; (D) IL-6; (E) MCP-1 transcription and production induced by LPS. Expression of the target genes were measured by q-PCR and normalized against the expression of GAPDH. Target genes expression in untreated cells was normalized to 1. The secretions of NO, cytokines and chemokine in RAW 264.7 culture supernatant were determined by Griess reagent and ELISA, respectively. LPS: 100 ng/mL. Data are mean ± SEM value of three independent experiments (*P < 0.05; **P < 0.01; by unpaired t test).
