Figure 2
From: Prognostic relevance of UCH-L1 and α-internexin in pancreatic neuroendocrine tumors
Representative examples of validated protein expression in PNETs and paired pancreatic specimens. Part a: IHC results. Left panel: HE staining of tumors and their paired pancreatic specimens; middle panel 1: expression of UCH-L1, MAP1B and VCAN in tumor #121, #52 (upper) and #292, respectively; middle panel 2: HE staining of tumors and their paired pancreatic specimens; right panel: negative expression of UCH-L1 in tumor #31 with a few positive cells, positive expression of MAP2 and CaSR in tumor #257 and #52, respectively; lower panel: expression of PDX-1 in insulinoma #36 and the negative expression of PDX-1 in tumor #63. Scale bar: 100 µm. Part b: Western blot results. Upper panel: expression of UCHL-1 in 4 PNETs, reduced expression of UCH-L1 in para-tumour tissue (#5 N) and no expression of UCH-L1 in 3 normal pancreatic specimens; upper middle panel 1: expression of CDK4 in 4 PNETs, 1 para-tumor tissue (#5 N) and 2 normal pancreatic specimens, no expression of CDK4 in 1 normal pancreatic specimen (Nor #3); lower middle panel 2: expression of CaSR in 2 PNETs and no expression of CaSR in 2 PNETs, para-tumor tissue (#5 N) and 3 normal pancreatic specimens; lower panel: β-actin was used as internal control. The identification of tumor was shown in Supplementary Table S1.
