Figure 3
From: Ligand chain length drives activation of lipid G protein-coupled receptors
Functional response of S1P receptors to S1P synthetic analogs. Aequoscreen CHO-K1 cells expressing the different S1P receptors were subjected to stimulation with increasing concentrations of S1P-derived ligands and the resulting luminescence was measured. (A) S1P1, n = 4 (B) S1P2 from CHO-Aeq cells, n = 3 (C) S1P2 from S1P5 cells, n = 4 (D) S1P4, n = 4 (E) S1P5, n = 2. The corresponding dose-response curves for one representative experiment are shown, where each curve represents the mean ± S.E.M of duplicate data points. Luminescence intensities are normalized on maximal response to the natural agonist S1P. Histograms on the right show maximal efficacy (Emax) values calculated for each S1P analog at S1P receptors, based on the sigmoidal fitting of aequorin functional response. The data plotted represent the mean ± S.E.M and are expressed in % of maximal efficacy in response to stimulation by S1P. Statistical significance was assessed using one-way ANOVA with a Scheffe’s post hoc test: *p < 0.05, **p < 0.01, ***p < 0.001.
