Figure 4
From: Ligand chain length drives activation of lipid G protein-coupled receptors
NF-κB activation via S1P2 in response to S1P analogs. Human BEAS-2B cells naturally expressing S1P2 receptor were subjected to stimulation with increasing concentrations (10 nM to 2 µM, from light yellow to dark red) of C16, S1P and C19 and resulting NF-κB activation was followed by luminescence (luciferase reporter). Untreated cells were used as a control (in grey) and 1 µM condition was also performed after pre-incubation with the selective S1P2 antagonist JTE013 (in blue). Histograms represent the mean values ± S.E.M. on 3 independent experiments for each condition, expressed as a percentage of 1 µM S1P response. Statistical significance between maximal activation levels (plateau at 1–2 µM) for different compounds was assessed using one-way ANOVA with a Scheffe’s post hoc test: *p < 0.05.
