Figure 2
Macrophage released cytokines can synergistically induce mesenchymal phenotypic changes in TGF-β treated IEOs. (A) A schematic illustration of the OEMT model with IEOs co-cultured with RAW264.7 cells. (B) Quantitation of TNF-α in the culture medium from TGF-β treated IEOs with or without RAW264.7 cells or anti-TNF-α neutralizing antibody. (C) Representative images showing the IEOs following TGF-β treatment and RAW 264.7 cells. Bars, 100 μm. (D) The expression of α-SMA and Ki67 was examined in TGF-β1 treated IEOs with RAW 264.7 cells. Bars, 50 μm. (E) The percentage of α-SMA positive colonies was calculated. Data are presented as the mean ± SEM; n = 4 samples per bar, **p < 0.01, *p < 0.05. (F) The expression of vimentin and F-actin reorganization was examined in TGF-β1 treated IEOs with RAW 264.7 cells. Bars, 50 μm.
