Figure 6
TNF-α promotes mesenchymal phenotypic changes in TGF-β1 treated IEOs by regulating gene expression associated with EMT. (A) Representative images of the IEOs following TNF-α, TGF-β treatment with or without inhibitors. Bars, 100 μm. (B) The percentage of α-SMA positive colonies was determined. Data were presented as the mean ± SEM; n = 4 samples per bar, **p < 0.01. (C) The expression of α-SMA and Ki67 was examined in TGF-β1 treated IEOs with or without inhibitors. Bars, 50 μm. (D) Relative mRNA expression for EMT-related transcription factors was quantified after TNF-α treatment in IEOs. (E) Relative mRNA expression for EMT effector genes was quantified after TNF-α treatment in IEOs. Data are presented as the mean ± SEM; n = 3 samples per bar, *p < 0.05, control versus TNF-α or inhibitors; #p < 0.05, TNF-α versus inhibitors.
