Figure 7
Alteration of SMN levels does not affect FUS-induced eye degeneration in Drosophila. (a) Representative pictures showing eyes from flies expressing the indicated human genes under the control of GMR GAL4. Fly lines carrying UAS::flag-FUS wild-type or UAS::flag-FUSMM transgenes on the second chromosome, together with GMR-GAL4, were grown at 29 °C. Flies expressing UAS::flag-SMN on its own were crossed with the GMR-GAL4 driver, at 29 °C. wtFUS induces a very strong eye degeneration, while expressing either FUSMM or SMN separately determines only very mild alterations of eye morphology. Significantly, the simultaneous expression of flag-SMN does not affect the strong eye degeneration caused by wtFUS. (b) Representative pictures showing eyes from flies expressing the indicated human genes under the control of GMR GAL4. RNAi mediated downregulation of Smn gene expression in fly eyes (SMN_RI) was obtained by expressing a specific RNAi construct (P (TRiP.HMC03832) attP40) at 29 °C, in a hemizygous background for Smn (Df (3 L) SmnX7/TM6B).
