Figure 5
Loss of retinal ganglion cells and decreased retinal GRP75 expression in db/db mice. (A) Retinal whole mounts were stained for Brn3a to visualize the retinal ganglion cells (RGCs) and examined by confocal microscope. The density of RGCs was decreased in db/db mice. Scale bar = 50 μm. Data were shown as mean ± SD, n = 3. *P < 0.05. Student’s t test. (B–D) Immunostaining showing decreased GRP75 (B), ERp29 (C), and calreticulin (D) in db/db mouse retinas and controls. Scale bar = 50 μm for (B–D). Images represent results from 3 individual mice in each group. GCL: ganglion cell layer, INL: inner nuclear layer, ONL: outer nuclear layer. Fluorescence intensity was quantified by Image J software and expressed as fold of change relative to control (mean ± SD, n = 3). *P < 0.05. **P < 0.01, Student’s t test.
