Figure 2
From: Low-cesium rice: mutation in OsSOS2 reduces radiocesium in rice grains
Identification of the gene responsible for the decreased Cs+ uptake in lcs1. (a) Structure of OsSOS2 (Os06g0606000) and the mutation site in lcs1. (b) Genomic sequence of the mutation site: exon 11 of OsSOS2 is joined to the intergenic region. (c) Fragments of the genomic region containing the mutation site amplified using the marker primer set sosP1and sosP2 shown in (a). Het, an F1 plant derived from a cross between WT and lcs1. (d) RT-PCR analyses of OsSOS2 transcripts using the primers sosP3, sosP4, and sosP5 designed to recognize exons 9–11. RNA was extracted from the whole roots of 8-day-old seedlings. (e) RT-PCR analysis of transcripts using the primers sosP3, sosP4, and sosP6. (f) Codons and the amino acid sequence around the junction between exon 10 and the unspliced intron of OsSOS2 in lcs1. The FISL motif is a site for SOS3 binding.
