Table 1 Summary of the proteomic profile of peptides detected following BRIC126 immunoprecipitations in K562 cells, EXP and T0 erythroblasts, and mature erythrocytes (RBCs).

From: CD47 surface stability is sensitive to actin disruption prior to inclusion within the band 3 macrocomplex

Accession Number

Protein Description

K562 cells

EXP

T0

Mature RBCs

Total

IgG

Ratio

Total

IgG

Ratio

Total

IgG

Ratio

Total

IgG

Ratio

P60709

Actin, cytoplasmic 1

71

33

2.2

242

109

2.2

      

P61158

Actin-related protein 3

5

*

   

13

6

2.2

   

O43707

Alpha-actinin-4

8

*

59

20

3.0

86

45

2.0

   

Q562R1

β-actin like protein 2

14

3

4.7

45

19

2.4

      

Q96C19

EF hand containing protein D2

7

*

12

3

4

15

6

2.5

6

*

P15311

Ezrin

12

6

2

         

P47756

F-actin capping protein subunit β

7

1

7

17

6

2.8

      

P21333

Filamin A

42

15

2.8

54

6

9

      

Q08722

Leucocyte surface antigen, CD47

6

1

6

7

2

3.5

8

4

2

14

6

2.3

Q9UHB6

LIMA1

   

7

2

3.5

27

5

5.4

   

P26038

Moesin

22

7

3.1

3

1

3

      

O94832

Myosin-Id

12

2

6

         

P35579

Myosin-9

228

72

3.2

374

166

2.3

      

P35580

Myosin-10

154

37

4.2

         

P12829

Myosin light chain 4

13

2

6.5

8

1

8

26

5

5.2

   

P35241

Radixin

10

5

2

         

P02549

Spectrin α chain, erythrocyte

   

19

*

      

P11277

Spectrin β chain, erythrocyte

   

9

1

9

      

Q9NYL9

Tropomodulin-3

   

8

*

8

2

4

2

1

2

P06753

Tropomyosin α-3 chain

9

1

9

3

3

1

5

*

   

Q71U36

Tubulin α-1A chain

   

14

*

23

*

   

P62988

Ubiquitin

19

3

6.3

         

P68036

Ubiquitin-conjugated enzyme E2 L3

14

5

2.8

      

4

 

*

P08670

Vimentin

   

5

 

*

2

 

*

   
  1. The total Bric126 IP peptides and IgG peptides are shown alongside the ratio Total/IgG. Clean total peptides are underlined and bold. *Indicates no ratio as there were no IgG immunoprecipitated peptides.