Table 1 Comparison of the brightnesses of free AF488 dye and AF488-C(0)Aβ42 in 0.25 M and 5 M GdnHCl solution obtained from PCH analysis (Fig. 2).
sample | AF488 | AF488-C(0)Aβ42 | ||
---|---|---|---|---|
GdnHCl conc. [M] | 0.25 | 5 | 0.25 | 5 |
brightness [kHz] | 16.23 (±0.22) | 6.36 (±0.43) | 18.73 (±2.23) | 6.32 (±0.18) |