Figure 5
From: Anti-Aging Potentials of Methylene Blue for Human Skin Longevity
MB increases skin thickness and hydration. (A) Representative cross-section images from H&E staining of Epiderm EFT-412 tissues after MB treatment at various concentrations for two weeks. The three skin layers are pointed by colored arrows. Red: the stratum corneum layer, Blue: the keratinocyte layer and black: the dermis fibroblast layer. Scale bar = 200 μm. (B) Histogram plot comparing the thickness of fibroblast layer (black arrows in A) under different concentrations of MB treatment. The thickness of fibroblast layer was quantified using ImageJ on six consecutive slides for each skin tissue and the average thickness was shown. (C) Skin hydration assessed by DPM9003 device showing significantly escalated levels of hydration on the Epiderm EFT-412 skin tissues after two weeks of MB treatment at 0.5 and 2.5 μM. (*p < 0.05, **p < 0.01).
