Figure 3
NT-Sr suppress osteoclast formation and activity. (a,b) RAW264.7 cells were induced with 50 ng/mL RANKL on different samples for 5 d and detected by TRAP staining. TRAP-positive cells with at least three nuclei were considered osteoclasts. The number of osteoclasts was quantified. The scale bar represents 500 μm (a). (c) RAW264.7 cells were induced with 50 ng/mL RANKL on different samples for 3 d, and TRAP enzyme activity was measured at OD 405 nm. (d,e) BMMCs were cultured in the presence of RANKL (50 ng/mL) and M-CSF (30 ng/ml) for 7 d. The cells were then fixed and stained for the TRAP assay. TRAP-positive cells with at least three nuclei were considered osteoclasts. The number of osteoclasts was quantified. The scale bar represents 400 μm (d). (f) BMMCs were induced with 50 ng/mL RANKL and M-CSF (30 ng/mL) on different samples for 3 d, and TRAP enzyme activity was measured at OD 405 nm. *,**p < 0.05 and 0.01, respectively, compared with the Ti group, and #,##p < 0.05 and 0.01, respectively, compared with TiO2-NTs. At least three independent experiments were analysed, and the data are presented as the means ± SDs.
