Figure 4
NT-Sr inhibit osteoclast formation and function. (a,c) RAW264.7 cells were incubated with 50 ng/mL RANKL on different samples. After culturing for 5 d, the cells were fixed, F-actin ring staining was performed, and the cells were examined by fluorescence microscopy. The number of F-actin rings was quantified. The scale bar represents 500 μm (a). (b) RAW264.7 cells were cultured on the Corning Osteo Assay Surface and incubated with 50 ng/mL RANKL for 5 d. The medium was then changed to conditioned medium, and the cells were cultured with 50 ng/mL RANKL for another 3 d. The cells were removed, and the resorbed areas were observed under a scanning electron microscope. The scale bar represents 200 μm. (d) The resorption areas are presented as percentages of the total well areas. *,**p < 0.05 and 0.01, respectively, compared with the Ti group, and #,##p < 0.05 and 0.01, respectively, compared with TiO2-NTs. At least three independent experiments were analysed, and the data are presented as the means ± SDs.
