Figure 5
NT-Sr repress osteoclast-specific genes. RAW264.7 cells (a) and mouse BMMCs (b) were cultured on different samples and induced with 50 ng/mL RANKL and 30 ng/mL M-CSF (for BMMCs), and the total RNA was then collected. The relative mRNA expression levels of osteoclast-specific genes (TRAP, CK, MMP-9, and NFATc1) were assessed by qRT-PCR. For total protein extraction, RAW264.7 cells (c,e) and mouse BMMCs (d,f) were cultured on different samples in the presence of RANKL (50 ng/mL) and M-CSF (30 ng/ml, for BMMCs). The protein expression of osteoclast markers (TRAP, CK, and MMP-9) was then detected by immunoblotting. An antibody to β-actin was used as a loading control. A quantitative analysis of the band densities was performed, and the band densities were normalised to the loading control. Full-length blots are presented in Supplementary Figure 2. Sr1h and Sr3h represent NT-Sr1h and NT-Sr3h, respectively. *,**p < 0.05 and 0.01, respectively, compared with the Ti group, #,##p < 0.05 and 0.01, respectively, compared with TiO2-NTs, and %p < 0.05 compared with the NT-Sr1h group. At least three independent experiments were analysed, and the data are presented as the means ± SDs.
