Figure 6
NT-Sr inhibit RANKL-induced NF-κB activation. After RAW264.7 cells (a,b) and mouse BMMCs (e,f) were cultured on different samples for 3 d, the cells were stimulated with or without 100 ng/mL RANKL for 30 min, and the total proteins were extracted for western blot analysis. The expression of proteins in the NF-κB pathway and the levels of p-IKKβ, p-IκBα, and p-NF-κBp65 were detected. Antibodies against β-actin, total IKKβ, IκBα, and NF-κBp65 were used as loading controls. A quantitative analysis of the band densities was performed, and the band densities were normalised to the loading controls. RAW264.7 cells (c,d) were cultured on different samples for 3 d and stimulated with 100 ng/mL RANKL for 30 min. The nuclear extracts were then collected, and the DNA-binding activity of NF-κB was detected by electrophoretic mobility shift assay (EMSA). A quantitative analysis of the band densities was performed, and the band densities were normalised to the loading controls. Full-length blots are presented in Supplementary Figure 3. p-p65 and p65 represent p-NF-κBp65 and NF-κBp65, respectively; and the numbers 1, 2, 3 and 4 in the figure represent Ti, TiO2-NTs, NT-Sr1h and NT-Sr3h, respectively. *,**p < 0.05 and 0.01, respectively, compared with the Ti group, and #p < 0.05 compared with TiO2-NTs. At least three independent experiments were analysed, and the data are presented as the means ± SDs.
