Figure 7
NT-Sr promote RANKL-induced phosphorylation of ERK and inhibit the Akt/NFATc1 pathway. RAW264.7 cells were cultured on different samples for 3 d and stimulated with or without 100 ng/mL RANKL for 30 min, and the total protein was then collected for immunoblot analysis. (a) RANKL-induced phosphorylation of ERK, (b) p-Akt and NFATc1 were detected using β-actin, total ERK and Akt as loading controls. (c) A quantitative analysis of the band densities was performed, and the band densities were normalised to the loading controls. Full-length blots are presented in Supplementary Figure 4. The numbers 1, 2, 3 and 4 in the figure represent Ti, TiO2-NTs, NT-Sr1h and NT-Sr3h, respectively. *,**p < 0.05 and 0.01, respectively, compared with the Ti group, and #,##p < 0.05 and 0.01, respectively, compared with TiO2-NTs. At least three independent experiments were analysed, and the data are presented as the means ± SDs.
