Figure 2

LPS challenge induced an exacerbated inflammatory response in the Ptpn6 ^H-KO mice by modulating MAPK activation. Serum concentration of (A) TNF-α (B) IL-1β, (C) IL-6 and (D) nitric oxide in Ptpn6 ^H-KO and Ptpn6 ^f/f mice after LPS challenge (30 mg/kg body weight). Data are presented as mean ± SEM (n = 6 in each group) **P ≤ 0.05 and ***P ≤ 0.01. Liver levels of (E) iNOS and (F) phospho-JNK1/2, phospho-ERK1/2 and phospho-p38, and serum levels of (G) SAA, ApoE and LBP in Ptpn6 ^H-KO and Ptpn6 ^f/f mice after LPS challenge (30 mg/kg body weight). The blots shown here are cropped from full length gel. Full-length blots are included in the Supplementary Information file. (E) iNOS in Supplementary Information 1 (F) phospho-JNK1/2, phospho-ERK1/2 and phospho-p38 in Supplementary Information 2 and serum levels of (G) SAA, ApoE and LBP in Ptpn6 ^H-KO and Ptpn6 ^f/f mice Supplementary Information 3.