Figure 4 | Scientific Reports

Figure 4

From: Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water

Figure 4

Principal coordinates analysis (PCoA) of unweighted UniFrac distances comparing the β-diversity of mini-microbial communities of spiked water among DNA-, PMA-, RNA-based methods. The mini-microbial communities compositions were as follow: (a) all live cells of Bacillus amyloliquefaciens, Mycobacterium smegmatis, Escherichia coli and Yersinia enterocolitica; (b) all dead cells of the aforementioned species; (c) dead cells of B. amyloliquefaciens and M. smegmatis, and live cells of E. coli and Y. enterocolitica; (d) live cells of B. amyloliquefaciens and M. smegmatis, and dead cells of E. coli and Y. enterocolitica; determined using DNA-, PMA-, and RNA-based 16S rRNA MiSeq Illumina sequencing.

Back to article page