Figure 8
Metabolite changes in worms exposed to different bacterial diets. The effect of dietary intervention on worm metabolism was investigated by feeding N2 worms with three types of bacteria including E. coli OP50, E. coli HT115 and B. subtilis PY79. (a) FA composition of N2 worms fed with different bacterial diets. Worms fed an E. coli OP50 diet accumulated the highest abundance of C18:1 fatty acid. A relatively high abundance of C18:1 was also found in worms fed with the other E. coli strain HT115. Worms fed a B. subtilis PY79 diet accumulated odd chain fatty acids, such as C15:0 and C17:0. (b) FA composition of the three bacterial diets. The two E. coli bacterial strains were enriched with C14:0, C16:0 and C18:1, while B. subtilis PY79 bacteria were enriched with odd chain fatty acids C15:0 and C17:0. Polyunsaturated FAs were absent in all three bacterial strains. (c) AA composition of N2 worms fed with different bacterial diets. AA profiles of worms fed with different bacterial diets did not display marked changes. Worms fed the E. coli strains showed similar AA profiles, while feeding worms with a B. subtilis diet showed a high abundance of proline. (d) AA composition of the three bacterial strains. E. coli strains contained low levels of most AA species, except glycine, which is relatively high abundant in E. coli HT115. B. subtilis PY79 bacteria were enriched in many AA species, especially glycine, lysine and glutamic acid. (e) Principal Component Analysis (PCA) score plot showing group separation based on PL profiles in N2 worms fed with different bacterial diets. Worms fed a B. subtilis PY79 diet were clearly separated from those fed with an E. coli diet, and a distinctive separation was evident between worms fed OP50 or HT115 E. coli. (f) PL composition in N2 worms fed with different bacterial diets. The metabolites contributing most to the groups’ separation based on PC1 and PC2 of the PCA are shown. Bar graphs are expressed as mean ± SD. See also Supplementary Fig. S5 and Suplementary Table S1.
