Figure 2

Up-regulating ETBR gene expression in the BLA with ETBR lentiviral activation particles (LV-ETBR) attenuated anxiety-related behaviors. Knocking down ETBR gene expression in the BLA increased anxiety-related behaviors, and down-regulating both ETBR and ET1 had no additional anxiogenic effect. (a) The time spent in the open arms and the time spent in the closed arms in the elevated plus maze test. (b) The latency time and the food intake of the mice in the novelty-suppressed feeding test. (c) The time spent in the center of the open field box. (d,e) The total path length and the speed in the open field test. (f) The time spent in the open arms and the time spent in the closed arms in the elevated plus maze test. (g) The latency time and the food intake of the mice in the novelty-suppressed feeding test. (h) The time spent in the central area in the open field test. (i,j) Locomotor activity in the open field test. (n = 16/group). *P < 0.05, **P < 0.01,***P < 0.001, two-way ANOVA test with one factor as repeated measure for 2a and 2f, one way ANOVA post hoc test for 2b–2e and 2g–2i.