Figure 2

Proliferating cell nuclear antigen (PCNA) immunostaining (A) In donor tissue from 10-wk-old rat (T0). Black arrows indicate PCNA-positive spermatogonia, red arrows indicate PCNA-positive Sertoli cells and, arrow heads indicate PCNA-positive spermatocytes. (B) In adult rat testis xenograft at 8-wk-post grafting. Note that no PCNA-stained cells are present inside the seminiferous tubules. Although, PCNA-stained cells in the peri-tubular region (arrows) and in interstitial space (arrow head) were observed. Expression of PCNA protein in (C) In immature donor tissue from 6-day-old rat (T0) and, (D) Immature rat testis xenograft at 8-wk -post grafting. Black arrows indicate PCNA-positive spermatogonia, red arrows indicate PCNA-positive Sertoli cells. In (D), arrow heads indicate PCNA-positive spermatocytes. (E) Percentage of PCNA- positive Sertoli cells and (F) Percentage of PCNA- positive germ cells in donor and grafted testes. Data are presented as mean ± SEM. Bars with different letters are significantly different at P < 0.05. Scale bar = 50 µm.