Figure 1 | Scientific Reports

Figure 1

From: Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue

Figure 1

Obese omental adipose tissue microenvironment promotes IL-17 release. (A) IL-17+ and IL-22+ cells were quantified by FACS analysis of blood-derived memory CD4+ T cells cultured for 7 days without or with conditioned medium of omental adipose tissue (omCM) from lean (n = 3) or obese (n = 8) subjects. *P < 0.05. (B) IL-17 and IL-22 concentrations were determined by Luminex in blood-derived memory CD4+ T cells culture medium. *P < 0.05. (C) Correlation analysis between IL-17 concentration in blood CD4+ T cells culture medium and IL-1β concentration in omCM. The correlation coefficient (r) and p value were obtained by Spearman’s test. (D) IL17RA, IL22RA1 and IL1R1 mRNA were determined in pre-adipocytes (n = 7), CD31+ endothelial cells (n = 6) and adipocytes (n = 4) obtained from lipoaspirate adipose tissue samples. Data are shown as mean ± SEM. nd: not detected.

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