Figure 2 | Scientific Reports

Figure 2

From: Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue

Figure 2

Pro-inflammatory signature in human adipose tissue non-immune cells. (A) CCL2, CCL20, IL-8, IL-6 and pro-IL-1β mRNA were determined by RT-qPCR in pre-adipocytes (n = 10) and CD31+ endothelial cells (n = 6) cultured for 96 h and by QuantiGene Plex in adipocytes 3D cultured for 72 h (n = 10) without or with rIL-17 (10 ng/mL) or rIL-1β (10 ng/mL). *P < 0.05 versus untreated cells. # P < 0.05 versus rIL-17 treated cells. (B) Concentrations of CCL2, CCL20, IL-8 and IL-6 were quantified by Luminex technology in culture media of pre-adipocytes (n = 6), CD31+ endothelial cells (n = 6) and adipocytes (n = 7) in untreated conditions (white bars) and in response to rIL-17 (stripped bars) or rIL-1β (black bars). *P < 0.05 versus untreated cells. #P < 0.05 versus rIL-17 treated cells. Data are shown as mean ± SEM.

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