Figure 4
The timing of nucleus and nucleomorph DNA replication in the synchronous G. theta culture. (a) Change in the cell density in the culture synchronized by the 8-h light/16-h dark cycle. The error bar represents standard deviation of six technical replicates. (b) Fluorescent micrographs showing the EdU-labelled nucleus and nucleomorph. DIC, images of differential interference contact; DAPI, DNA stained with DAPI. The arrow and arrowhead indicate signals of nucleus and nucleomorph, respectively. Scale bar = 5 µm. (c) Change in the frequency of cells with EdU-labelled nucleus (blue), cells with nucleomorph (red), and cells with both EdU-labelled nucleus and nucleomorph (green). The error bar indicates standard deviation of three technical replicates.
