Figure 5 | Scientific Reports

Figure 5

From: Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta

Figure 5

Change in the level and localization of nucleomorph-encoded FtsZ in the synchronous G. theta culture. (a) Change in the cell density in the culture synchronized by the 8-h light/16-h dark cycle. The error bar represents standard deviation of six technical replicates. (b) Immunoblot analysis showing the change in the FtsZ protein level and the level of histone H3 that is phosphorylated at serine 10 (H3S10ph) in the synchronous culture. Coomassie Brilliant Blue (CBB) staining is shown as the loading control. (c) Immunofluorescent micrographs showing the localization of FtsZ in the synchronized cells. DIC, images of differential interference contact; Anti-FtsZ, nucleomorph-encoded FtsZ detected with the antibody; Chl, chloroplast autofluorescence; DAPI, DNA stained with DAPI; FtsZ/Chl, merged images of Anti-FtsZ and Chl. The double arrowhead indicates constriction of the chloroplast division site. The arrow indicates DAPI signal of the nucleus. Scale bar = 5 µm.

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