Figure 3

Immunostaining of E-cadherin, N-cadherin and ZO-1 in kidneys of untreated animals and after extended liver resection. E-cadherin (left panels, red), N-cadherin (middle panels, green) co-stains and ZO-1 (right panels) were detected by immunofluorescence in kidney tissue of untreated animals (top) and of animals after partial hepatectomy without (control, centre) or with application of pBM-MSC (bottom). Original magnification: 200x; DT = distal tubule; PT = proximal tubule. Arrows and solid arrowheads indicate membrane and cytoplasmic localization of N-cadherin, respectively. Open arrowheads point to discontinuous expression of ZO-1 after liver resection.