Figure 3

Knockdown of FAM3B inhibits high glucose-induced VSMC proliferation and migration. VSMCs were transfected with scramble (scra) siRNA (as negative control) or FAM3B siRNA for 48 h in DMEM medium containing 5.5 mM or 25 mM glucose. Cell proliferation was evaluated using the EdU incorporation (A,B) or direct cell counting assay (C). (D) Assessment of the cell cycle progression by flow cytometry. (E) Protein expression levels of key regulators involved in cell cycle progression. (F) Determination of VSMC migration by transwell chamber (top) and wound-healing (bottom) assays. (G,H) Quantification of the data from (F). (I) Protein expression levels of ICAM-1, VCAM-1, and MMPs. (J,K) Total activity of MMP-9 and MMP-2. ^** P < 0.01 vs. scra/5.5 mM glucose, ^## P < 0.01 vs. scra/25 mM glucose.