Figure 1

miR-150 deficiency decreases responsiveness to antigenic stimulation and antitumor activity of CD8⁺ T cells in vivo. (a) Kinetics of mir-150 ^+/+ and mir-150 ^−/− CD8⁺ T cells after antigenic stimulation in vivo. Thy1.1:TCR_gag:mir-150 ^+/+ or Thy1.1:TCR_gag:mir-150 ^−/− CD8⁺ T cells were transferred to Thy1.2 C57/BL6 mice. The mice were injected with Lm-gag intraperitoneally. The transferred CD8⁺ T cells were analyzed using blood samples at 0, 5, 7, 12, and 54 d after the transfer. (b) Expression of surface markers in mir-150 ^−/− and mir-150 ^+/+ CD8⁺ T cells at 5 d after transfer and Lm-gag injection. (c) Relative mRNA amount of activation-induced molecules in mir-150 ^−/− and mir-150 ^+/+ CD8⁺ T cells after stimulation for 5 h. (d) Antitumor activity of mir-150 ^−/− and mir-150 ^+/+ CD8⁺ T cells in vivo. Mice were injected intra-peritoneally with FBL-gag cell. At 5 d after FBL injection, the mice were received cytoxan (Cyt) and 6 h later transferred mir-150 ^+/+ or mir-150 ^−/− CD8⁺ T cells. FBL; FBL injection, Cyt; FBL and Cyt injection, mir-150 ^−/− or mir-150 ^+/+; FBL, cyt and CD8⁺ T cell injection. *P < 0.05, **P < 0.01, ***P < 0.001. Data are means ± SEM of duplicate triplicate samples from a single experiment and are representative of two independent experiments.