Table 1 Error rate estimates from two independent experiments. The table shows the number of mismatches that remain after consensus sequence assembly for reads tagged with the same UMI, the total number of unique UMI tags observed, and error rate estimates for 20 (25 for Phusion) cycles of PCR amplification with a 150-bp template. Error rate estimates are provided as the number of erroneous bases per template base per cycle, with 95% confidence intervals (CI) calculated using normal approximation for binomial proportions. Error rate^LA represents estimates for the linear amplification step, while Error rate^corr represents the estimate of conventional PCR error rate after correcting for linear amplification errors.

Polymerase*	Exp.	Error count	UMI tags	Error rate, x10 ⁻⁵ [95% CI]	Error rate ^LA , x10 ⁻⁵	Error rate ^corr , x10 ⁻⁵ [95% CI]
Encyclo	1	24557	185560	4.30 [4.25, 4.35]	12.79	3.68 [3.63, 3.73]
Encyclo	2	14211	101516	4.55 [4.48, 4.62]	12.79	3.93 [3.86, 3.99]
Kapa HF	1	339	7876	1.40 [1.25, 1.55]	8.17	1.00 [0.88, 1.13]
Kapa HF	2	2519	57052	1.44 [1.38, 1.49]	8.17	1.04 [0.99, 1.08]
Phusion	1	30	1348	0.72 [0.47, 0.98]	4.99	0.48 [0.27, 0.69]
Phusion	2	23	1351	0.55 [0.33, 0.78]	4.99	0.31 [0.14, 0.48]
SD-HS	1	6714	33076	6.60 [6.46, 6.74]	26.89	5.29 [5.16, 5.42]
SD-HS	2	10362	58518	5.76 [5.66, 5.86]	26.89	4.45 [4.36, 4.54]
SNP-detect	1	457	13870	1.07 [0.97, 1.17]	5.04	0.83 [0.74, 0.91]
SNP-detect	2	848	32310	0.85 [0.80, 0.91]	5.04	0.61 [0.56, 0.66]
Taq-HS	1	1875	15137	4.03 [3.86, 4.20]	18.36	3.13 [2.98, 3.29]
Taq-HS	2	3113	24082	4.20 [4.07, 4.34]	18.36	3.31 [3.18, 3.43]
Tersus-buffer1	1	2550	46927	1.77 [1.70, 1.83]	5.98	1.48 [1.41, 1.54]
Tersus-buffer1	2	5504	154226	1.16 [1.13, 1.19]	5.98	0.87 [0.84, 0.89]
Tersus-buffer2	1	6282	130891	1.56 [1.52, 1.60]	5.1	1.31 [1.28, 1.35]
Tersus-buffer2	2	1299	30683	1.38 [1.30, 1.45]	5.1	1.13 [1.06, 1.19]
TruSeq	1	312	14164	0.72 [0.64, 0.79]	4.1	0.52 [0.45, 0.58]
TruSeq	2	362	16705	0.70 [0.63, 0.78]	4.1	0.50 [0.44, 0.57]
KTN	1	16802	132733	4.12 [4.06, 4.17]	12.92	3.49 [3.43, 3.54]
KTN	2	6298	44331	4.62 [4.51, 4.73]	12.92	3.99 [3.89, 4.09]

^*Encyclo (Evrogen JSC), Kapa HiFi PCR Kit (Kapa Biosystems), Phusion High-Fidelity DNA Polymerase (NEB), SD-HS²⁹, SNP-detect, HS Taq, Tersus in buffer 1 (Mg²⁺ 3.5 mM, Ph 8.5), Tersus in buffer 2 (Mg²⁺ 2.5 mM, Ph 8.0) (Evrogen JSC), TruSeq Custom Enrichment Kit (Illumina), KTN (KlenTaq N polymerase)³⁰.

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