Table 2 Frequency of substitution types produced during 20 PCR cycles.
From: A high-throughput assay for quantitative measurement of PCR errors
Polymerase | A > C, T > G | A > G, T > C | A > T, T > A | C > A, G > T | C > G, G > C | C > T, G > A |
|---|---|---|---|---|---|---|
Encyclo | 4% | 59% | 5% | 2% | 1% | 29% |
Kapa HF | 2% | 10% | 2% | 14% | 1% | 71% |
SD-HS | 3% | 31% | 14% | 5% | 17% | 30% |
SNP-detect | 5% | 27% | 1% | 2% | 1% | 64% |
Taq-HS | 3% | 56% | 7% | 7% | 2% | 25% |
Tersus-buffer1 | 5% | 35% | 2% | 6% | 1% | 52% |
Tersus-buffer2 | 5% | 45% | 2% | 2% | 1% | 46% |
TruSeq | 6% | 13% | 3% | 25% | 3% | 50% |
KTN | 4% | 60% | 4% | 2% | 1% | 29% |
