Figure 3

Steroidogenic-pathway enzyme deregulation and estradiol decrease in mice exposed to the mixture of EDCs. (A) Testosterone and estradiol levels of seminiferous tubular fluid were measured by RIA, two individual measures per testis (right and left) run in duplicate. (B) RT-qPCR of genes implicated in the steroidogenic process. (C) Cyp19a1 expression levels in embryonic gonads of male mice on post-coital day (dpc) 14.5, postnatal day (dpn) 3, and adult mouse testes at postnatal day 60. All graphics represent the mean ± SEM, n = 4. Unpaired t test, *p < 0.05, **p < 0.01. Abbreviation: AU, arbitrary units.