Figure 1
From: MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys
(A) A schematic diagram illustrating the experimental paradigm. (B) Immunostaining and H&E staining of pancreatic biopsies from normal monkeys (a, d), diabetic monkeys 3 months after STZ injection (b, e) and diabetic monkeys up to one year after implantation (c, f). In diabetic monkeys, insulin expression (red fluorescence) decreased, whereas glucagon expression (green fluorescence) appeared to have expanded. H&E staining showed that exocrine pancreatic cells in diabetic monkeys appeared histologically normal. (C) After 8 passages, the PSCs maintained classic MSC-like morphology, and immunofluorescence labeling showed that the PPCs were positive for nestin, Isl-1 and vimentin but negative for PDX-1 and insulin. (D) The PPCs exhibited a cell surface marker expression pattern similar to that of bone marrow-derived MSCs. Flow cytometry analysis showed that the PPCs were positive for CD105, CD73, and CD90 but negative for CD45, CD34, and HLA-DR. Scale bars represent 100 μm.
