Figure 1

Characterization of 4.5 dpc tetraploid blastocysts. (A) Differential expression of NANOG in diploid and tetraploid embryos at the 4.5 dpc blastocyst stage. Bar scale = 20 μm. (B) Percentages of different groups of blastocysts. Diploid blastocysts and tetraploid blastocysts were differentially stained with EPI, and total cells were individually classified into four groups according to the number of EPI-positive cells (0%, 0–5%, 5–10% and 10%). 0% represented no epiblast cells in the blastocyst, 0–5% represented the percentage of epiblast cells in the blastocyst greater than 0% and less than or equal to 5%. 5–10% represented the percentage of epiblast cells in the blastocyst greater than 5% and less than or equal to 10%. 10%- represented the percentage of epiblast cells in the blastocyst greater than 10%. (C) The methylation status of the Oct4 promoter showed differentially methylated regions in diploid and tetraploid blastocysts. (D) The colonies of diploid and tetraploid ESCs were round and three-dimensional. Bar scale = 200 μm.