Figure 2 | Scientific Reports

Figure 2

From: Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues

Figure 2

Characterization of tetraploid ESCs. (A) Tetraploid ESCs were positive for AP staining. Bar scale = 200 μm. (B) Immunocytochemical staining of OCT4, SOX2 and SSEA1 in tetraploid ESCs. Bar scale = 10 μm. (C) Hematoxylin and eosin staining of teratoma sections of tetraploid ESCs. Left: blood vessel of endothelium (ectoderm); middle: muscle (mesoderm); right: gut-like epithelium (endoderm). Scale bars = 50 μm. (D) Karyotype analysis of diploid and tetraploid ESCs. (E) Methylation status of the Oct4 promoter showed methylated regions in diploid and tetraploid ESCs. (F) Relative mRNA expression levels of pluripotent genes in diploid and tetraploid ESCs. Different superscripts represent statistically significant differences between groups (P < 0.05).

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