Figure 2 | Scientific Reports

Figure 2

From: MicroRNA-34a Encapsulated in Hyaluronic Acid Nanoparticles Induces Epigenetic Changes with Altered Mitochondrial Bioenergetics and Apoptosis in Non-Small-Cell Lung Cancer Cells

Figure 2

MicroRNA-34a induced cellular apoptosis. Dose-dependent and time-dependent analyses of genes responsible for induction of apoptotic signaling and simultaneous inhibition of anti-apoptotic genes, post transfection with HA-PEI/HA-PEG-nanoparticles encapsulated miR34a on (A) wild-type A549 and (B) cisplatin resistant A549DDP cells. Normalized to beta-actin and untreated control samples using relative quantification by delta(delta(Ct) method. (C) Caspase 3 activity levels measured using ELISA assay in both A549 and A549 DDP cells. Blank NPs–Blank HA-PEI/HA-PEG nanoparticles, miR34a HA-NPs–microRNA34a encapsulated in HA-PEI/HA-PEG nanoparticles. *Indicates comparison against control = 1. One-way ANOVA followed by Post-hoc t-test with multiple comparisons, *p < 0.05, N = 6.

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