Figure 7

Effect of miR34a transfection with HA-based nanoparticles (50 nM; 24 hours) on mitochondrial bioenergetics and mitochondrial genes encoded on nuclear DNA. A quantitative real time polymerase chain reaction (qRT-PCR) assay was used to measure the levels of PGC1alpha, NRF1 and NRF2 with the (A) wild-type A549 cells and (B) cisplatin resistant A549DDP cells post-transfection with HA-PEI/HA-PEG-nanovectors encapsulated miR34a, normalized to beta-actin RNA and untreated control samples using relative quantification by delta(delta(Ct) method. (C) Lactate dehydrogenase mRNA levels were measured using a qRT-PCR. (D) Lactate activity was measured using an ELISA assay. (E) Ratios of reduced to oxidized Glutathione levels measured using HPLC. (F) Mitochondrial and Cytoplasmic ATP levels measured using ELISA assay. Blank NPs–Blank HA-PEI/HA-PEG nanoparticles, miR34a - Naked miR34a without encapsulation, miR34a HA-NPs–microRNA34a encapsulated in HA-PEI/HA-PEG nanoparticles. Results are expressed in units (mean +/− standard error of mean [SEM]); *indicates comparison against control = 1. One-way ANOVA followed by Post-hoc t-test with multiple comparisons, *p < 0.05, **p < 0.01; n = 6.