Figure 4

Relative quantification of cell-free supernatants (Bacteriocin units (BU)/ml). Antimicrobial activity of (a) bactofencin A and (b) pediocin PA-1 produced by E. coli transformed with the plasmids APC2313 (control), APC2315, APC2318, APC2321 and APC2322, and APC2658, APC2663, APC2664 and APC2666, respectively, in comparison with the samples from the natural producers Lb. salivarius DPC6502 (Bfn) and P. acidilactici LMG2351 (Ped). *Bacteriocin activity (BU/ml) was calculated as the inverse of the highest dilution showing inhibition of indicator strains divided by volume (0.05 ml).