Figure 8
Detection of native peptide, PB-TUP fused P2 and BSA conjugated P2 by ELISA. (A) Binding property of P2 and PB-TUP-P2 was studied by direct ELISA using these two peptides as capture agents. P2 absorbed weakly to polystyrene surfaces, while the fused peptide PB-TUP-P2 showed a strong binding ability to polystyrene in a concentration dependent manner. (B) BSA conjugated P2 was used as coating agent and P2 specific antibody was used to investigate the binding of BSA-P2. We used the dilution from 1:100 to 1:5000 and not any difference was found from the ELISA value.
