Figure 4

NKX6.3 negatively regulates NF-kB expression and activity. (A) NKX6.3 decreased the expression of NF-kB subunit p65 mRNA transcript in real-time PCR analysis. Results are represented as mean ± SEM from three independent experiments. (B) NKX6.3 reduced NF-kB subunit p50 mRNA expression in AGS and MKN1 cells. Results are represented as mean ± SEM from three independent experiments. (C) In AGS and MKN1 cells, NKX6.3 reduced expression of NF-kB p65 and p50 proteins in western blot analysis. (D and E) ChIP and ChIP-qPCR analyses of NKX6.3 binding to the promoters of NF-kB p65 (D) and p50 (E) genes. The results are represented as mean ± SEM from two independent experiments. Each dot plot represents the result from the individual experiment. **P < 0.01, * P < 0.05, based on the student’s t-test.