Table 1 Derived kinetic parameters for the conversion of asymmetric dimethylarginine to L-citrulline by dimethylarginine dimethylaminohydrolase 1.

From: Human dimethylarginine dimethylaminohydrolase 1 inhibition by proton pump inhibitors and the cardiovascular risk marker asymmetric dimethylarginine: in vitro and in vivo significance

Parameter

Replicate 1

Replicate 2

Replicate 3

Mean

SD

Km (µmol/L)

49.8

45.3

41.5

45.5

4.2

Vmax (pmol/min/mg)

378

355

326

353

26

F statistic

8307

4620

827

  

R-squared

0.9990

0.9981

0.9892

  
  1. Kinetic constants (K m , Vmax) for L-citrulline formation were derived from fitting the Michaelis-Menten equation to experimental data using the nonlinear curve fitting software EnzFitter. SD: standard deviation.
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