Figure 2 | Scientific Reports

Figure 2

From: Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection

Figure 2

The biomarker at m/z 400.3 was fully identified as palmitoylcarnitine (PalC). (A) Mass spectrum from MALDI-FTICR-MS showing the accurate mass of the biomarker at 400.3 (400.342). (B) A mass spectrum from the on tissue fragmentation of the molecule at m/z 400.3 and from the fragmentation of the standard by DESI-Orbitrap-MSI showing the similar fragmentation pattern. (C) Heat map images from DESI-orbitrap-MSI validating identification by showing localisation of parent ion (m/z 400.34) to the same area as the main fragment from PalC (m/z 85.03). Colour scale bar shows the colour gradient of the MSI image in relation to relative abundance of the imaged molecule, colour gradient from 0% relative abundance of molecule to 100% relative abundance.

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